Coding

Part:BBa_K4343101

Designed by: Yue Shu   Group: iGEM22_NNU-China   (2022-10-09)


PaDes12

PaDes12 encodes ∆-12 desaturase from Parietichytrium sp. which functions in the desaturase and elongase pathway and could convert C18:1 to C18:2.

Description

The PaDes12 gene encodes a protein of 399 amino acids. Heterologous expression of PaDes12 could catalyze C18:1 to C18:2 in Y. lipolytica.

Yohei Ishibashi et al. applied this enzyme in C20ELO KO cells to obtain a 6-fold higher yield of the target product, demonstrating the high availability of this enzyme activity.

Reference

Ishibashi, Y., Goda, H., Hamaguchi, R., Sakaguchi, K., Sekiguchi, T., Ishiwata, Y., ... & Ito, M. (2021). PUFA synthase-independent DHA synthesis pathway in Parietichytrium sp. and its modification to produce EPA and n-3DPA. Communications Biology, 4(1), 1-11. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 1217


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